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Role of Syk-Cys in phagocytosis. A , the ΔSyk Raw cell line does not express Syk. Western blot of cells following Syk or Firefly luciferase knockout in RAW cells using <t>CRISPR/Cas9.</t> B , Phagocytosis efficiency of Cys to Ser mutants of mSyk following transfection of EGFP-mSyk in ΔSyk RAW cells. Cells were allowed to phagocytose IgG-opsonized 3µm-diameter latex beads for 15 min at 37°C, before counting beads inside and outside cells. See Figure S1 for details.
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Role of Syk-Cys in phagocytosis. A , the ΔSyk Raw cell line does not express Syk. Western blot of cells following Syk or Firefly luciferase knockout in RAW cells using <t>CRISPR/Cas9.</t> B , Phagocytosis efficiency of Cys to Ser mutants of mSyk following transfection of EGFP-mSyk in ΔSyk RAW cells. Cells were allowed to phagocytose IgG-opsonized 3µm-diameter latex beads for 15 min at 37°C, before counting beads inside and outside cells. See Figure S1 for details.
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Role of Syk-Cys in phagocytosis. A , the ΔSyk Raw cell line does not express Syk. Western blot of cells following Syk or Firefly luciferase knockout in RAW cells using <t>CRISPR/Cas9.</t> B , Phagocytosis efficiency of Cys to Ser mutants of mSyk following transfection of EGFP-mSyk in ΔSyk RAW cells. Cells were allowed to phagocytose IgG-opsonized 3µm-diameter latex beads for 15 min at 37°C, before counting beads inside and outside cells. See Figure S1 for details.
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Role of Syk-Cys in phagocytosis. A , the ΔSyk Raw cell line does not express Syk. Western blot of cells following Syk or Firefly luciferase knockout in RAW cells using <t>CRISPR/Cas9.</t> B , Phagocytosis efficiency of Cys to Ser mutants of mSyk following transfection of EGFP-mSyk in ΔSyk RAW cells. Cells were allowed to phagocytose IgG-opsonized 3µm-diameter latex beads for 15 min at 37°C, before counting beads inside and outside cells. See Figure S1 for details.
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Role of Syk-Cys in phagocytosis. A , the ΔSyk Raw cell line does not express Syk. Western blot of cells following Syk or Firefly luciferase knockout in RAW cells using CRISPR/Cas9. B , Phagocytosis efficiency of Cys to Ser mutants of mSyk following transfection of EGFP-mSyk in ΔSyk RAW cells. Cells were allowed to phagocytose IgG-opsonized 3µm-diameter latex beads for 15 min at 37°C, before counting beads inside and outside cells. See Figure S1 for details.

Journal: bioRxiv

Article Title: Syk activation during FcγR-mediated phagocytosis involves both Syk palmitoylation and desulfenylation

doi: 10.1101/2025.05.26.656092

Figure Lengend Snippet: Role of Syk-Cys in phagocytosis. A , the ΔSyk Raw cell line does not express Syk. Western blot of cells following Syk or Firefly luciferase knockout in RAW cells using CRISPR/Cas9. B , Phagocytosis efficiency of Cys to Ser mutants of mSyk following transfection of EGFP-mSyk in ΔSyk RAW cells. Cells were allowed to phagocytose IgG-opsonized 3µm-diameter latex beads for 15 min at 37°C, before counting beads inside and outside cells. See Figure S1 for details.

Article Snippet: To prepare ΔSyk cells we used the Addgene all-in-one CRISPR/Cas9 vector (#79144) as described ( Giuliano et al , 2019 ).

Techniques: Western Blot, Luciferase, Knock-Out, CRISPR, Transfection